Cover of: Confocal Microscopy of the Cornea | Leonardo Mastropasqua

Confocal Microscopy of the Cornea

  • 144 Pages
  • 1.64 MB
  • English
Slack Incorporated
Medical imaging, Ophthalmology, Diseases And Disorders Of The Eye, Diagnosis, Medical / Nursing, Medical, Examination, Confocal microscopy, Medical / Optometry, Cornea, Diagnostic use, Dis
The Physical Object
ID Numbers
Open LibraryOL8595377M
ISBN 101556426119
ISBN 139781556426117

This work describes confocal microscopy of the cornea, a new imaging diagnostic technique in ophthalmology. Chapters review the principles of confocal microscopy, describe various types of corneal pathologies, look at the application of the procedure during corneal surgery, and discuss postoperative follow-up and identification of complications.

This work describes confocal microscopy of the cornea, a new imaging diagnostic technique in ophthalmology. Chapters review the principles of confocal microscopy, describe various types of.

The aim of the following study is to compare in vivo laser scanning confocal microscopy (IVCM) qualitative investigation of the ocular surface, including conjunctiva, cornea, limbus, blebs and sclera, in POAG patients who underwent the Xen 45 Gel Stent implant, trabeculectomy or medical by: 5.

- Buy Confocal Microscopy of the Cornea book online at best prices in India on Read Confocal Microscopy of the Cornea book reviews & author details and more at Free delivery on qualified : Leonardo Mastropasqua, Mario Nubile.

A confocal microscope is a type of microscope in which a thick object such as the cornea is illuminated with a focused spot of light.

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The same microscope objective that is used to illuminate a point, P1, in the object is used to collect the scattered and reflected light from same point P1. This is illustrated in Fig. by: Laser-Scanning in vivo Confocal Microscopy of the Cornea: Imaging and Analysis Methods for Preclinical and Clinical Applications.

By Neil Lagali, Beatrice Bourghardt Peebo, Johan Germundsson, Ulla Edén, Reza Danyali, Marcus Rinaldo and Per Fagerholm. Submitted: June 20th Reviewed: November 23rd Published: March 20th DOI: /Cited by: To collect and quantify 3-D information from the cornea, a technique termed confocal microscopy through-focusing (CMTF) was developed for the TSCM by Jester and coworkers.

40,41 This technique is based on the observation that different corneal sub-layers generate different reflective intensities when imaged using confocal microscopy.

42 CMTF scans are obtained by scanning through the cornea Cited by:   Aims To evaluate the impact of herpes simplex virus (HSV)-induced scar location on bilateral corneal nerve alterations using laser in vivo confocal microscopy (IVCM).

Methods Central and peripheral corneal subbasal nerve density (CSND) were assessed bilaterally in 39 patients with unilateral HSV-induced corneal scars (21 central scars (CS), 18 peripheral scars (PS)) using IVCM.

Description Confocal Microscopy of the Cornea PDF

The first scanning confocal microscope was developed by Minsky in 74 Conventional light microscopes produce poor quality images due to increased reflections and scattered light from structures outside of the focal plane.

77 The confocal microscope actively eliminates the light from the planes outside the focal point, as those rays do not fall directly onto the detection aperture. In vivo laser confocal microscopy enables to visualize normal human cornea layer by layer: superficial epithelial cells, basal epithelial cells, Bowman’s layer with nerves, Bowman’s layer with K-structures (Kobayashi-structures), stroma and endothelial cells (Figure 2).

[ 5, 6] K-structures are fibrous structures with a diameter of 5 to 15 µm, and are considered to be anterior collagen fiber. The availability of confocal microscopy in the clinic provides an opportunity to study cornea changes after contact lens wear.

Confocal microscopy can be performed over the contact lens to observe changes in corneal cellular morphology. Studies have shown that contact lens wear causes stromal acidosis and hypoxia.

Aims. To investigate changes in corneal nerve morphology in Type 2 diabetes and to establish relationships between in vivo corneal confocal microscopy and markers of peripheral nerve structure and function.

Participants and methods. We recruited 57 participants with Type 2 diabetes and 26 healthy controls of similar age and sex distribution. Confocal microscopy provides the capacity for direct, noninvasive, serial optical sectioning of intact, thick, living specimens with a minimum of sample preparation as well as a marginal improvement in lateral resolution compared to wide-field microscopy.

The purpose of this study was to evaluate whether corneal confocal microscopy can identify early corneal nerve damage and change in LC density in.

Corneal confocal microscopy is a rapid, noninvasive technique that quantifies axons with good sensitivity and specificity.

11 We have established corneal nerve fiber density as a measure of degeneration and corneal nerve branch density as a measure of regeneration that may increase the utility of CCM as an imaging end point in clinical trials of novel agents in MS.

The procedure of confocal microscopy enables ophthalmologists to examine the cornea with extraordinary resolution and magnification without the invasiveness common in other procedures. This resource explores the procedure. Described within its chapters are the principles of confocal microscopy, the various types of corneal pathologies, the application of this procedure during corneal.

The aim of the current study was to evaluate the distribution and morphology of corneal nerves as seen by means of white light confocal microscopy. Methods. This study analyzed images of corneal nerves that were obtained using the Tomey Confoscan slit scanning confocal microscope (40×/ objective lens).

The images were classified according to their location within the cornea. Confocal microscope is an ophthalmic instrument that provides real-time and non-invasive serial imaging of cornea.

With the principle of both projecting of light to the tissue and focusing lens have the same focal point, it has a high magnification of 40X to visualize many layers of cornea, in sequence, the epithelial layer, subepithelial nerve plexus (which has not demonstrated by any.

f1: In vivo confocal microscopy of the cornea and the limbus in a normal eye with the an in vivo laser confocal microscope with an attachment for the cornea (Heidelberg Retina Tomograph II–Rostock Cornea Module, or HRTII-RCM®; Heidelberg Engineering, Heidelberg, Germany).

Panels A through F represent areas of × µm corresponding to the superficial epithelial cell layer, wing cell.

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We discuss techniques for performing 3-D imaging with the HRT-RCM, including hardware and software modifications that allow full-thickness confocal microscopy through-focusing (CMTF) of the cornea, which can provide quantitative measurements of corneal sublayer thicknesses, stromal cell and extracellular matrix backscatter, and depth-dependent changes in corneal keratocyte density.

The stability of the corneal surface following keratoplasty confirms that the renewal of the corneal epithelium was effectively retained.

Examination of the corneal surface by in vivo confocal microscopy (IVCM) allows for objective identification of corneal and conjunctival cell phenotypes to evaluate different epithelialization patterns.

Abstract. In vivo, scanning-slit, confocal microscopy offers improved resolution and has resulted in new discoveries of corneal pathology at the cellular ability to provide high resolution, real-time images of the full thickness of theliving human cornea gives.

Confocal slit-scanning video-microscopy is a very useful technique for in vivo investigation of corneal nerve regeneration after PRK and, presumably, other keratorefractive procedures. Confocal reflectance microscopy has demonstrated the ability to produce in vivo images of corneal tissue with sufficient cellular resolution to diagnose a broad range of corneal conditions.

To investigate the spectral behavior of corneal reflectance imaging, a modified laser ophthalmoscope was used. Imaging was performed in vivo on a human cornea as well as ex vivo on porcine and lamb corneae.

Confocal Microscopy of the Cornea by Leonardo Mastropasqua, MD and Mario Nubile, MD is intended to provide the reader with a brief description of the clinical and research possibilities of confocal microscopy of the cornea.

The book is divided into 11 chapters. Abstract. Background: Corneal nerves can be examined using in vivo confocal microscopy (IVCM). This new technique permits sequential observation of the corneal subbasal nerve plexus and detects early signs of diabetic peripheral neuropathy.

Patients with the rare genetic disorder Fabry’s disease—a lysosomal storage disorders—have particular ocular needs. Specifically, they’re at elevated risk for corneal dystrophy.

1 Traditionally, these patients are evaluated for epithelial deposits using a slit lamp, but with the development of in vivo corneal confocal microscopy (IVCM), researchers are wondering if this. Specular microscopy is a noninvasive photographic technique that allows you to visualize and analyze the corneal endothelium.

Using computer-assisted morphometry, modern specular microscopes analyze the size, shape and population of the endothelial cells. The instrument projects light onto the cornea and captures the image that is reflected from the optical interface between the corneal.

This educational video shows how to set up Corneal Confocal Microscopy (CCM), acquire corneal nerve fiber images in the central cornea and inferior whorl. Results: In healthy volunteers, in vivo confocal microscopy revealed LCs in 31% of all volunteers, with 37 of these 43 volunteers presenting LCs both in the center and the periphery of the cornea with densities of 34 ± 3 and 98 ± 8 cells/mm 2, respectively.

In the group of contact lens wearers, 55% of all corneas presented with LCs, and 11 of. We carried out an investigation into the morphological and quantitative corneal properties in dry eye with various underlying pathologies. Ten patients with aqueous tear deficiency, 8 with dysthyroid ophthalmopathy, 8 with chronic lagophthalmos and 10 normal participants were examined.

Confocal microscope images were taken at the centre and at the lower and upper periphery of the cornea. Corneal confocal microscopy (CCM) is a non-invasive ophthalmic technique that has been used to quantify corneal nerve fibers as a surrogate for small fiber pathology (SFP) in a .‎Welcome back to EyePod Bayer!

This exciting episode will be the first in a series of interesting interviews and discussions about imaging of the eye. We will start in the anterior part, with confocal microscopy of the cornea, and continue with imaging of the back of the eye and retina with OCT and O.